It was a tale of two penalties and a late winner for the Blues in Lancashire as striker Kyle Soper completed a dramatic turnaround to grab the win for the visitors in what was an entertaining game on Tuesday afternoon.
The young Blues had the bulk of the possession, and the chances, and they nicked the win late in the day thanks to a crisp finish from United's first year forward.
Rovers took the lead from the spot, only to have it cancelled out by a Carlisle penalty, courtesy of a cool finish from Josh Dixon.
Youth boss Darren Edmondson said: "This was very encouraging and the lads will have taken a lot from it.
"Billy Barr has got a very good side together at Blackburn, they did well in the FA Cup last year, but we matched them for energy levels and application and we played some good football along the way.
"They took the lead, probably deservedly so, but we stuck at it and we created a number of very good chances. Once Josh equalised with his penalty you could see us grow in confidence even more and we're pleased for Kyle that he got his goal to win the game.
"We had two very good one-on-one situations which would have wrapped things up completely, but it was probably tiredness of mind, as much as anything else, which meant they were missed."
"The down side from the day is that we picked up a couple of injuries," he added. "Josh Dixon had to be substituted, and one of our under-16 lads took a knock, but hopefully they'll both recover quickly.
"We had a few other under-16 lads with us and it was a great experience for them. Everybody played well and it was good for us as coaching staff to see the things we've talked about coming together during match time."
Next up for the youth team is the trip to face Derby County on Saturday [21 July], kick off 1pm.